An artificial non-coding RNA module created by a synthetic biological method and a use thereof in construction of an artificial nitrogen fixation system. The RNA module enhances the stability of a nitrogenase coding gene nifHDK mRNA after transcription by interacting with the nifHDK mRNA, thereby improving the nitrogen fixation capability of chassis microorganisms. A fusion expression vector of the artificial RNA module is constructed and transferred into different chassis nitrogen fixation microorganisms. Experiments prove that under the condition of nitrogen fixation, the artificial RNA module can remarkably improve the activity of recombinant engineering strain nitrogenases.
